Effect of Different Concentrations of IAA (Indole acetic acid) and IBA (Indole butyric acid) on Multiple Root Regeneration of Banana (Musa spp.) cv. Giant Cavendish from Meristem Derived Explants

The present study was conducted at the Tissue culture Laboratory of Amhara Region Agricultural Research Institute (ARARI), Bahir Dar - Ethiopia during the period from May to June 2012 to investigate the effect of different concentrations of IAA and IBA on root regeneration of banana cv. Giant Cavendish. For rooting, individual shoots (3-6 cm) with 5-6 leaves were transferred to the MS medium supplemented with (0.0, 0.5 and 1.0 mg/l) IAA and (0.0, 0.5 and 1.0 mg/l) IBA for 4 weeks. Among the different concentrations, highest number of roots was produced by 0.5 mg/l IAA + 0.5 mg/l IBA (3.00, 4.67, and 6.67) 10, 20, and 30 DAI respectively. The longest roots observed was (2.83, 4.60, and 5.87 cm) at 10, 20, and 30 DAI respectively in concentration 0.5 mg/l IAA + 0.5mg/l IBA, which was statistically significant (P<0.05) at 10, 20, and 30 DAI.